?Methods?Limbal stem cells were cultured by tissue patch and examined with colony forming efficiency(CFE)and expression of keratin 3 in normal corneal tissue and cultured corneal epithelial cells on coverslip by immunofluorescent staining.

• 方法组织块法培养细胞，测定细胞克隆形成率（CFE），免疫荧光染色检测干细胞表达角质蛋白K3的状况。