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- 1,4-ben zoquinone-N-benzoylhydra zone oxime敌菌腙
- 作者用黄曲霉毒素B_1(AFB_1)与羧甲基羟胺半盐酸盐(CMO)在碱性介质中回流反应,使AFB_1生成AFB_1羧甲基肟(AFB_1-oxime),将此衍生物偶联到牛血清白蛋白(BSA)上,制得人工抗原AFB_1-oxime-BSA。Carboxymathyloxime of afla-toxin B_1 (AFB_1)was synthesized by refluxingAF B_1 with carboxymethoxylamine hemi-hydrochloride for 4 hours in the presenceof pyridine. AF B_1-BSA(antigen) was madesuccessfully by coupling AF B_1-oxime withbovine serum albumin(BSA) in the presenceof a water soluble carbodiimide. The rab-bits were immunized with the artificialimmunogen which had been purified by di-alysis.