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- 目的 建立携带人类淀粉样前体蛋白瑞典型突变 (Swedish mutation of amyloid precursorprotein,APPSWE)基因的转基因小鼠模型。Objective To prepare transgenic mice model of human (Swedish mutation of amyloid precursor protein, APP SWE ) gene. Methods By pronuclear microinjection, human APP SWE genes were microinjected into pronuclei of zygotes of C57 and Kunming white mice.
- 目的 确认淀粉样纤维蛋白基因 (amyloid fibrils,BRI)基因在 1对同源但转移能力不同的肺腺癌细胞系 AGZY83- a和 Anip973中的序列并分析其表达。Objective To investigate the sequence of amyloid fibrils (BRI) gene and its expression in two lung adenocarcinoma cell lines AGZY83-a and Anip973 with the same tumor origin but different metastatic potential.
- 近年许多实验证实C-型利钠肽(c-type natriuretic peptide, CNP)及其受体广泛存在于中枢神经系统(central nervous system, CNS)。Recently, many scholar have found that c - type natriuretic pep-tide (CNP) and the receptor - CNP(R - CNP) in the central nerve system (CNS).
- 降钙素基因相关肽(Calcitonin gene-related peptide,CGRP)是由降钙素基因编码的mRNA选择性拼接后转录而成的37个氨基酸组成的单链多肽，是全身最主要的扩张血管的神经活性多肽。Calcitonin gene-related peptide (CGRP) composed of 37 amino acids by alternative splicing of messenger RNA transcript of calcitoini gene.
- PAC2是垂体腺苷酸环化酶激活多肽(Pituitary adenylate cyclase activating polypeptide,PACAP)和血管活性肠肽(vasoactive intestinal peptide,VIP)的共同受体,介导多种重要生物学功能。VPAC2 is a co-receptor of pituitary adenylate cyclase activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) and mediates multiple bio-functions. In order to construct the CHO line expressing VPAC2 stably,pcDNA-VPAC2 was used to transfect CHO cells.
- 抗嗜铬素A(Chromogranin A，CgA)、抗神经元特异性烯醇化酶(Neuron specidic-enolase，NSE)记DNES细胞，抗生长抑素(Somatostatin，SS)和抗血管活性肠肽(Vasoactive intestnal peptide，VIP)检测DNES细胞的内含物。Neuroendocrine cells were identified by immunohistochemical staining of chromogranin A (CgA) and neuron specific enolase (NSE). Somatostatin (SS) and vasoactive intestinal peptide(VIP) were used to identify the inclusions of neuroendocrine cells.
