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- VH和VL重组质粒分别转化到大肠肝菌BL21(DE3)中,IPTG诱导后,经SDS?PAGE电泳表明在12kD处有包含体蛋白表达,表达蛋白含量分别为28%和35%。The recombinaint plasmids of VH and VL which were transformed into E. coli BL21 (DE3) separately produced a 1.2 kD inclusion body protien upon induction with IPTG. The expression level of VH(and VL) protein was 28%25 (and 35%25).
- 在高pH时分子皿为26,500的包含体蛋白被酶降解为19,500和15,600道尔顿的两个组分。 Hg++、Cu++仅部分抑制酶活力,可被二异丙基氟磷酸(DFP)完全抑制。In high pH, it hydrolyzed the major constituent of the inclusion body protein, a 26,500-dalton protein (granulin), 10 two polypeptides with molecular weights of 19,500 and 15.600, ft displayed higher activity at pH 9.4 and was partialy inhibited by Hg++ and CU++, and completely inhibited by diisopropyl phosphorofluridate.
- 包含include
- 蓖麻蛋白A链与单抗3G11偶联物的制备及体外生物活性研究The preparation of immunoconjugate composed of ricin A-chain and mAB 3G11 and the evaluation of its biological activity in vitro
- 胞浆内包含体intracytoplasmic inclusion
- 嗜铬蛋白Achromograin A
- 包含体,小体cytorrhyctes
- 周期蛋白Acyclin A
- 包涵体,包含体IB; inclusion body
- 外膜蛋白Aouter membrane protein A (OmpA)
- 利普许茨包含体Lipschutz's bodies
- 机械通气对新生儿气道灌洗液中肺表面活性物质蛋白A的影响Influence of mechanical ventilation on tracheal aspirate fluid surfactant- associated proteins- A levels in neonates
- 沙鼠包含体因子Sand rat inclusion agent
- 淀粉样前体蛋白的原核表达、纯化及抗血清的制备和应用Expression and Purification of Recombinant Human APP Proteins in E. Coli and Preparation of Rabbit Antiserum Against APP
- 人扭转蛋白Ahuman torsin A
- 无包含体杆状病毒non-occluded baculovirus(NOB)
- 载脂蛋白AⅠAApolipoprotein A Ⅰ
- May-Hegglin包含体May-Hegglin inclusion
- 原生质育种筛选可利用泔水发酵生产菌体蛋白饲料的研究Study on protoplasm breeding to screen bacteria for swill fermentation to produce microbiology protein feeds
- 载脂蛋白AⅤApolipoprotein A-V
