1) Three binary expression vectors were constructed: 1) p13W4H, containing loxcDNA hairpin structure controlled by endosperm specific promoter (ESP) and hpt gene;

• 1) 构建了三个用于转化的双元表达载体：A：p13W4H，含胚乳特异表达启动子、lox cDNA发夹结构及潮霉素选择标记基因hpt；