3) After biopsied single lymphocyte amplificed with nested PCR and PEP-nested PCR, the allele dropout (ADO) rate of SRY gene by nested PCR and PEP-nested PCR were 15.63%, 3.13%, while amplification rate were 93.06%, 98.61%;

• SRY基因的脱扣率分别为15.；63%25；3