A PET32a vector containing human OGT cDNA-S tag was expressed in BL21 E. Coli and the recombinant protein was purified with S-protein conjugated agarose beads.

• 携带人 OGTc DNA的 PET32 a质粒在大肠杆菌中表达出带有 1个 S- Tag的重组人 OGT融合蛋白 ,然后用与 S-蛋白质偶联的琼脂糖凝胶颗粒从细菌裂解液中亲和纯化 OGT融合蛋白。