A full-length cDNA of DFL and its antisense nucleotide sequence have been transformed into Nicotiana tabacum under the control of a cauliflower mosaic virus 35S promoter.

• 为了鉴定DFL基因的功能作用,构建了由35S启动DFL基因的正反义表达载体(载体中同时包含有由另外两个35S启动的潮霉素筛选基因和GUS组织化学染色基因)转化烟草。