A new HPLC method with fluorescence detector was developed and validated for the simultaneous determination of dextrorphan and guaifenesin in human plasma.

• 建立了灵敏度高、选择性好、可同时测定人血浆中右美沙芬活性代谢物去甲右美沙芬及愈创木酚甘油醚的HPLC-荧光检测法。