A pair of primers of thrombomodulin in rat for PCR reaction were designed and studied.The PCR reactions were optimized by adjusting the dose of primers or template and the renaturation temperature.

• 摘要设计了大鼠血栓调节蛋白基因PCR扩增引物，并对引物与模板的加入量及退火温度等进行了研究，优化了PCR反应。