A pair of specific primer was designed for amplification of cry2Ab gene. The amplified products of cry2Ab gene was directly inserted into pUCm-T vector and transformed into E.

• 根据已知的cry2Ab基因ORF序列，设计合成了一对特异性引物，PCR扩增出cry2Ab基因全长，并直接与pUCm-T载体相连。