A system used for the determination of fidelity of DNA polymerase in PCR was developen in E.coli and was used to determine the fidelity of FD DNA polymerase in PCR amplication.

• 摘要 在大肠杆菌中建立了一套用于测定 DNA聚合酶在PCR过程中复制精确性的系统,并测定了耐热FD DNA聚合酶在PCR扩增过程中的复制精确性。