A thrombin-like enzyme (TLE) was isolated and purified from the venom of Agkistrodon acutus by ion exchange chromatography on DEAE-Sephadex A-50 and gel filtration on Sephadex G-75.The TLE(1.5U/kg) was given intravenously to anesthetized dogs .

• 本文借助 DEAE-sephadex A-50和 Sephadex G-75柱层析从尖吻蝮蛇毒(AAV)内分离并纯化出一种类凝血酶(TLE).