According to NHS and MA methods, TS hapten was cross linked to BSA (OVA).Artificial immunogen and coating were synthesized.UV-scanning was used for preliminary qualitation of conjugates.
英
美
- 采用混合酸酐法、碳二亚胺法合成人工免疫原、包被原,通过紫外扫描法对其偶联结果进行初步鉴定,结合免疫小鼠及新西兰白兔的试验,间接竞争ELISA监测表明多抗血清针对磺胺类药物产生特异性抗体,确证偶联成功。