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- The construction of recombinant adenoviral vector containing gene of EWS-FLI1 and its expression in PBMC . 介导尤文肉瘤融合基因的重组腺病毒的构建及其在外周血单个核细胞(PBMC)中的表达
- Methods rVSMCs were transfected with an adenoviral vector expressing Mfn2(Adv-Mfn2-GFP). 方法体外培养rVSMCs,感染含有Mfn2基因的腺病毒载体(Adv-Mfn2-GFP)。
- Objective:To evaluate the effects of endostatin adenoviral vector on malignant ascites. 目的:探讨内皮抑素基因治疗在癌性腹水中的治疗作用。
- This study was aimed to investigate the transfection efficiency of adenoviral vector AD5/F35 to hematopoietic malignant cells lines of various origins and AD5/F35 cytotoxicity. 摘要本研究比较AD5/F35腺病毒载体对不同来源的血液系统恶性细胞系感染效率的差异和细胞毒性反应。
- Objective Rabit bone marrow stromal cells(BMSC) infected by a recombinant adenoviral vector carrying the human BMP 2 gene(Ad BMP 2) were seeded into BCB scaffolds to construct tissue engineering bone in vitro. 目的 用人骨形态发生蛋白 2腺病毒表达载体 (Ad BMP 2 )转染的兔骨髓基质干细胞 (BMSC) ,种植BCB(去抗原牛松质骨 )支架体外构建组织工程骨。
- SERCa2a mRNA greatly expressed in the SERCa2a gene treatment group and SERCa2a gene modified MSC transplantation group than that in the MSC transplantation group and the empty adenoviral vector control group. 肌浆网钙离子ATP酶2a基因治疗组和肌浆网钙离子ATP酶2a基因修饰的骨髓干细胞移植治疗组心肌内肌浆网钙离子ATP酶2amRNA表达强度明显高于骨髓干细胞移植治疗组和腺病毒空载体对照组。
- AIM:To study the effect of transgenic of bone morphogenetic protein 2 adenoviral vector Ad BMP 2 on the cultivation in vitro of compound of fibrin gel and mesenchymal stem cell (MSC). 目的:研究骨形态发生蛋白-2重组腺病毒(Ad-BMP-2)转基因对骨髓基质细胞(mesenchymalstemcell,MSC)与纤维蛋白凝胶构成的复合物体外培养的影响。
- The effect of 12C6+ beam irradiation on AdCMV-GFP(a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. 探讨12C6+离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒(AdCMV-GFP)转染小鼠黑色素瘤细胞(B16细胞系)的影响。
- Conclusion: Adenoviral vector can effectively transfect VEGF gene into NIH3T3 cells;VEGF gene can be detected in vitro and in vivo;and it can promote neovascularization in the transplanted tissues. 结论:腺病毒介导的VEGF 基因可有效转染NIH3T3细胞,体内外均可有效表达目的基因,并可促进移植组织血管新生。
- helper-dependent adenoviral vector 辅助病毒依赖型腺病毒载体
- replication-defective adenoviral vector 复制缺陷型腺病毒载体
- The genes which code for said proteins are transferred to the mammary epithelial cells using adenoviral vectors. 编码所述蛋白质的基因通过腺病毒载体转移到乳腺上皮细胞内。
- replication-deficient adenoviral vector 复制缺陷型腺病毒载体
- RESULTS: Recombinant adenoviral vectors containing melittin gene were successfully constructed, and it was melittin gene that could be transcripted proved by RT-PCR. 结果:携蜂毒素基因的重组腺病毒载体构建成功,RT-PCR实验表明蜂毒素基因可以得到转录。
- AIM: To construct the recombinant adenoviral vector carrying antisense RNA to chemokine receptors CCR5 and CXCR4 and to obtain recombinant adenovirus, which will be used to resist HIV 1 infection. 目的 :构建趋化因子受体CCR5 ;CXCR4双靶区反义RNA重组载体并获取重组腺病毒以用于抗HIV 1基因治疗的研究 .
- Objective To test the anti-tumor effect of adenovirus vector generated from the homologous recombination of bacteria mediating TK gene on hepatic neoplasm cell. 目的探讨用细菌内同源重组法制备含TK自杀基因腺病毒对肝癌细胞的杀伤作用。
- The dispersed pancreas cells were transfected with exogenous gene by adenovirus vector and cationic polymer reagent, respectively. 在细胞分散后对孔鳐胰脏细胞用腺病毒载体和阳高子多聚物进行外源基因的转染。
- The results showed that: adenovirus vector in HEK-293 cells in the packaging successful, the virus appears macrophages spot. 2. 最后用磷酸钙转染法把重组腺病毒载体转染到 HEK-293细胞中包装。
- Results: Adeno-X-SSTR2,recombinant adenovirus vector,can infect pancreatic carcinoma cells MIA-PaCa-2 and AsPC-1 efficiently,which both can express SSTR2 mRNA and their protein. 在不同浓度NC-8-12和奥曲肽作用下;未转染SSTR2基因胰腺癌细胞株的Survivin表达量无明显变化(P>0.;05);转染了SSTR2基因胰腺癌细胞株的Survivin表达量明显下调(P<0
- Results Through direct infection and MTT assay,we found that adenovirus vector containing angiostatin K1-5 could inhibit the proliferation of human vascular endothelial cells. 结果直接感染台盼蓝染色排除法及MTT法检测表明,携带血管抑素K1-5基因的腺病毒能显著抑制血管内皮细胞增生,并对其具有杀伤力。