After 1,4,7,10,14 days of exposure,the rats were killed,whole lung RNA were isolated and cytochrome b mRNA were detected with reverse transcription polymerase chain reaction(RT-PCR).

• 两组分别于高氧或空气暴露后1、4、7、10和14d时提取肺组织RNA,采用半定量逆转录聚合酶链反应(RT-PCR)测定Cytb mRNA表达;