After 14 additional hours in the dark, one retina of each quail was collected for HPLC analysis, and the contralateral retina was embedded in paraffin for counts of apoptotic nuclei.

• 14小时后，对每个组员的一个视网膜进行HPLC分析，对侧的视网膜则植入石蜡以计算调亡的细胞核。