After PCR analysis of To individuals, we found the transformation frequency of co-transformation plasmid pUC-MT2-15 and pACH20 was higher than pCAB2-15. After Southern hybridization, 4 T_0 individuals were confirmed being transformed by 1By15 gene.

• 在对1By15的基因枪法转化中,共转化质粒pUC-MT2-15和pACH20的转化效率明显高于pCAB2-15。 Southern杂交证明有4个转基因品系(T_0)有1By15基因的插入;