After electrophoresis of lysate from different tissues in SDS-PAGE and transferring into the NC membrane, we used the antiserum to RAP in Western Blot Analysis.

• 分别提取大鼠各组织蛋白，经蛋白质聚丙酰胺凝胶电泳（SDS－PAGE）分离后，转移至硝酸纤维素膜，用抗RAP抗血清进行蛋白质印迹分析（WesternBlotAnalysis）。