After that, DES hemisuccinate was binded to bovine serum albumin (BSA) by the method of mixed acid anhydride, at the same time, the production was analyzed by SDS-PAGE and calculated the number of DES Hapten.

• 再利用混合酸酐试验将己烯雌酚半琥珀酸结合到牛血泊白蛋白(BSA)上,对反应产物进行SDS·PAGE鉴定,计算BSA分子上结合的己烯雌酚半抗原数目;