All samples infected by CMV,LMoV and LSV could be amplified by multiplex PCR,and yielding three special bands of CMV(657 bp),LMoV(428 bp)and LSV(171 bp).

• 该方法可以从带有CMV、LMoV和LSV的样品中同时扩增出3条大小与试验设计相符的657bp(CMV)、428bp(LMoV)、171bp(LSV)的特异性多重RT-PCR扩增带。