Apoptosis of brain cells was identified with terminal eoxynucleotidyltransferse mediated dUPT-biotin nick end labeling (TUNEL) and the response intensity of bcl-2 protein was evaluated with immunohistochemical staining.

• 实验结束后快速断头取脑,石蜡切片,采用DNA末端标记技术(TUNEL法)观察各组脑细胞凋亡的变化情况并采用免疫组化染色评定bcl-2蛋白反应强度。