Based on the position weight matrix(PWM) and length distribution of open reading frame(ORF),a simple method for predicting translation initiation sites is presented. It can identify TIS from upstream AUGs easily.

• 结合位置权重矩阵(PWM；position weight matrix)和开放阅读框架(ORF；open reading frame)的长度分布特征建立了简单的方法识别翻译起始位点；此方法能很好地区分上游AUG和TIS.