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- Secondly, we prepared the bone marrow mononuclear cell suspension using the method of Ficoll cell separation, which separate、purify and culture the bone marrow monocytic cell. 2. 制备骨髓单个核细胞悬液，利用Ficoll密度梯度离心分离法，分离、纯化骨髓单个核细胞，培养单个核细胞。
- Expression of PTTG mRNA was detected in bone marrow mononuclear cell (BMMNC) from 32 cases with MDS and 10 normal controls by reverse transcriptase-polymerase chain reaction (RT-PCR). [方法]采用RT鄄PCR方法检测32例MDS患者及10例正常骨髓单个核细胞(bonemarrowmononuclearcell,BMMNC)中PTTGmRNA的表达。
- In this study,The mRNA expression of VLA-4 in bone marrow mononuclear cells was measuerd by RT-PCR. 研究采用逆转录-聚合酶链反应(RT-PCR)法检测慢性再障患者治疗前后骨髓单个核细胞VLA-4的mRNA表达水平。
- AIM: To explore the amount of bone marrow mononuclear cells migrating to the liver during allografting in mice. 结论: 骨髓干细胞向肝脏迁移的细胞数与移植途径无关.
- Objective To define in vitro whether bone marrow mononuclear cells (BM MNC) secrete vascular endothelial growth factor (VEGF) and to evaluate the effect of BM MNC on endothelial cell proliferation. 目的:探讨体外培养骨髓细胞分泌促血管生长因子的能力及培养上清对内皮细胞增殖的影响。
- The patients were divided into trial group[PCI+normal saline(NS)] and control group[PCI+autologous bone marrow mononuclear cells(BM-MNCs)] randomly. 随机分为对照组[PCI+生理盐水(NS)]与试验组[PCI+自体骨髓单核细胞(BM-MNCs)]。
- Methods Bone marrow mononuclear cells derived from CML were cultured in RPMI-1640 supplemented with GM-CSF and IL-4 for 7 days. 方法分离慢性粒细胞白血病(CML)患者骨髓单个核细胞,加入GM-CSF和IL-4诱导培养7d,将所得细胞分为4组:完整失活流感疫苗(WIV)组;
- Autologous bone marrow mononuclear cell 自体骨髓单个核细胞
- Methods A real time quantitative PCR(RQ-PCR) was performed to measure the bcr/abl~(P190) transcripts in the bone marrow mononuclear cells from 74 CML patients in different phases. 方法应用实时定量聚合酶链反应(RQ-PCR)检测74例CML患者骨髓单个核细胞中的bcr/ablP190转录本。
- Methods:Bone marrow mononuclear cells(MNCs) from 10 newly diagnosed Ph chromosome positive CML patients in chronic phase were separated and cultured in vitro for 10 days. 方法 :分离 10例初诊的慢性期Ph+ CML患者骨髓单个核细胞 (MNC) ,体外培养 10天 ,利用染色体分析法检测培养前后Ph+ 中期细胞比例 ;
- OBJECTIVE: To discuss the influence of allogenic bone marrow mononuclear cells (BM-MNCs) transplantation to the myocardial apoptosis of rats and related gene expression after AMI. 目的：探讨同种异体骨髓单个核细胞移植对急性心肌梗死大鼠心肌细胞凋亡及相关基因表达的影响。
- The chimerism and functional expression of mdr1 gene in bone marrow mononuclear cells were detected by polymerase chain reaction (PCR), Streptavidin-Peroxidase immunohistochemistry (IC) staining anddaunorubicin (DNR) efflux assay in the different aspects. 采用PCR方法、免疫组化染色法、柔红霉素排出试验分别从基因、蛋白质和功能水平检测mdr1基因在兔骨髓单个核细胞中的功能性表达情况，探讨转染时间和转染效率、功能之间的关系。
- In the group of mice exposed to 10Hz magnetic field,the weight of mice was measured,the quantity of bone marrow mononuclear cells and CFU-S(colony forming unit-spleen) was also detected. 以10Hz旋转磁场作用于137Cs放射损伤小鼠,检测磁场组与对照组体重、骨髓有核细胞数及脾结节数。
- Methods: The expression rates of p-gp and Bcl-2 in bone marrow mononuclear cells(BMMNC) from 97 patients with AL and 26 patients with CML were detected by flow cytometry with direct immunofluorescent labeling. 方法：应用流式细胞仪（FCM），采用直接免疫荧光标记法对97例AL和26例CML患者骨髓单个核细胞（BMMNC）进行P-gp和Bcl-2蛋白检测。
- Cultural supernatant of bone marrow mononuclear cell(CSBMMNC) 骨髓单个核细胞培养上清
- Bone marrow mononuclear cells (BM-MNCs) 骨髓单个核细胞
- bone marrow mononuclear cells (BMMNC) 骨髓单个核细胞
- SDF-1 combined with bone marrow mononuclear cell transplantation for the treatment of hindlimb ischemia in rats 基质细胞衍生因子联合骨髓单个核细胞移植治疗大鼠肢体缺血的实验研究
- Keywords Bone marrow mononuclear cell;Cell transplantation;Ventricular remodeling;Myocardial infarction;Dog; 骨髓单个核细胞;细胞移植;心室重构;心肌梗死;犬;
- Effects of allograft bone marrow mononuclear cell implantation on rat cardiac myocyte apoptosis and expression of apoptosis-associated genes after acute myocardial infarction 同种异体骨髓单个核细胞移植对急性心肌梗死后大鼠心肌细胞凋亡及相关基因表达的影响
