By immunohistochemical study, the bone morphogenetic protein(BMP) content in callus was calculated by computerized interacive morphometry (CIM) in the 1st, 2nd, 3rd, 4th, 5th week after fracture.

• 于骨折后第1，2，3，4及5周用断颈法每次每组处死5只兔，取骨痂进行抗骨形态发生蛋白（BMP）单克隆抗体免疫组化染色，并用CMIAS型图像分析仪对BMP含量进行定量分析。