By using two-hybrid system,the bait plasmid containing ipaC gene was constructed and designated pGBKT-IpaC. A human HeLa cDNA library was screened to isolate protein factors that might interact with IpaC.

• 首先将IpaC全长基因克隆到诱饵蛋白载体pGBKT7中 ,以构建的pGBKT IpaC为靶蛋白质粒 ,筛选人HeLa细胞cDNA文库。