CONCLUSIONS: This novel real-time enterovirus assay is a sensitive and suitable assay for routine clinical testing. The presence of single nucleotide polymorphisms can affect real-time PCR assays.

• 结论：这种实时定量PCR的方法是足够敏感和稳定满足常规的临床样本分析的。但是单核苷酸多态性的出现能影响到实时定量PCR的检测结果。