Callus was induced using seeds as explant on Dioscorea nipponica Makino,the best culture media is MS+6-BA3.0 mg/L+NAA2.0 mg/L and the highest frequency is 18.9%.

• 以穿龙薯蓣种子为外植体诱导出了愈伤组织；最佳培养基为MS+6-BA3.；0mg/L+NAA2