Care should be taken to ensure that steam used for sterilisation is of suitable quality and does not contain additives at a level which could cause contamination of product or equipment.

• 如果灭菌的产品不是在密封的容器里，要用能够排除空气并让蒸汽渗入，但能够防止灭菌后再污染的材料进行包装。所有的装载的产品，都要与灭菌剂在规定的温度下接触达到规定的时间。