Cell cycle was detected by PI assay. Flow Cytometry was used to detect the P-glycoprotein on the surface of the cells, the intracellular concentration of DNR, and the immuetype of the cells.

• 细胞毒实验采用MTT法 ,细胞周期测定采用碘化丙啶荧光染色法 ,细胞表面P -糖蛋白 (P -gp)、细胞内柔红霉素 (DNR)浓度、以及细胞免疫表型测定采用流式细胞术测定。