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SCs were isolated by warm enzyme digestion, tissue culture, cold enzyme digestion of substep and cold enzyme digestion of combine. 雪旺细胞的分离采用了组织块法、热酶消化法、冷酶分步消化法和冷酶联合消化法。
Cold enzyme digestion 冷酶消化法
The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes. 酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
Epithelial depuration was carried out by integration of burning and enzyme digestion. 细胞形态观察和电镜检测鉴定细胞。
Both enzyme digestion and alkali treatment are effective methods of cell rupture, but each has a major disadvantage. 酶消化法和碱处理法都是细胞破碎的有效方法，但是也都有各自的缺点。
Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
The obtained bands were cloned into pGEM T-Easy vector, and the positive clone were confirmed by EcoR I enzyme digestion. 将所获片段克隆入pGEN T-Easy载体，EcoR I酶切鉴定。
Methods By tryosin and DNA enzyme digest methods, we observed the human beings chorial trophcytes with light microscope and electronic microscope. 方法应用胰蛋白酶／DNA酶消化法进行消化，经光镜和电镜观察。
The recombination vector that was named as pCAM-P was obtained based on the tests of PCR and enzyme digestion of the vector. 2. PCR检测、质粒酶切分析结果表明获得了Pto与pCAMBIA1300的重组子，命名为pCAM-P。
Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. 利用酶切、AT克隆方法快速分析筛选出保护性抗原基因片段的重组子,从而制备成芯片探针。
BACKGROUND: At present, the preparation methods of acellular vascular matrix include enzyme digestion and decontaminant. 背景：目前脱细胞血管基质的制备方法有酶消化法和去污剂法。
The cells were cultured by explant culture and enzymatic digestion. 组织化学染色和异种移植。
Alkali treatment is the antithesis of enzyme digestion, for it is harsh rather than gentle, nonselective rather than specific, and cheap rather than expensive. 碱处理法和酶消化法相反，反应激烈，不具选择性，而且较便宜。
Results 1. The result of restriction enzyme digest and gene sequence showed that the EWS-FLI-1 binding sequence (ACCGGAAGT) lay at the upper stream of diphtheria toxin A fragment. 结果 1、对重组载体的酶切鉴定和测序结果证实，在pS2-DTA中EWS-FLI-1特异性结合序列(ACCGGAAGT)位于白喉毒素A链基因上游。
Results 1.Both GAP-43 ORF gene fragment and the recombinant AAV-EGFP-GAP-43 plasmid were verified by enzyme digestion and DNA sequencing for correct reading frame and orientation. 结果:.;1
Methods Exon 7 of SMN was detected in 15 cases with SMA and 33 cases of nomal control with polymerase chain reaction-restriction enzyme digestion analysis. 方法用聚合酶链反应-酶切技术对15例SMA病人及33例正常对照的外显子7进行检测，明确有无缺失。
Then insert the fusion gene CTB-ureB into the vector pUC19. The recombinant plasmid was identified with PCR and enzyme digestion, and was confirmed by sequence analyze. CTB、ureB基因的融合及克隆：采用高保真PCR技术，分别从质粒pGEM-CTB和幽门螺杆菌基因组DNA中扩增出CTB和cagA基因，然后通过PCR法融合两个基因，最后将CTB-ureB融合基因插入载体pUC19，PCR筛选、酶切鉴定阳性克隆，进行DNA序列分析验证。
Methods Polymerase chain reaction with restrict enzyme digestion was used to detect the target gene polymorphism in 78 normotension controls and 72 EH patients. 目前认为，高血压是多种遗传因素和环境因素共同作用而引起的复杂性状疾病，在人类遗传易感性的基础上，在环境危险因素的作用下发病。
This strain of wheat can grow during a cold spring. 这种小麦可以在寒冷的春天生长。
Conclusion: The enzyme digestion method can effectively isolate mesenchymal stem cells with a high proliferative ability and multipotent differentiation ability. 结论：酶消化法可有效的从胎盘组织中分离间充质干细胞，增殖能力强，具备多向分化能力。