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- Colorectal neoplasm Lovo cells 大肠癌Lovo细胞
- Methods:Using RT-PCR, we detected the E-cadherin and N-cadherin on Lovo cells of human colorectal cancer under the effect of cisplatin,pirarubicin, mitomycinC, and 5-FU at different concentration and acting time . 方法 :应用逆转录聚合酶链反应 (RT -PCR)法检测大肠癌Lovo细胞E -Cad herin ,N -Cadherin的表达 ,并通过几种不同的化疗药物 (顺铂、吡柔比星、丝裂霉素、5 -FU)不同浓度和时间作用后 ,对E -Cadherin ,N -Cadherin表达的影响。
- human colorectal carcinoma LoVo cell 人大肠癌LoVo细胞
- Background Regular use of aspirin reduces the risk of a colorectal neoplasm, but the mechanism by which aspirin affects carcinogenesis in the colon is not well understood. 研究背景：定期服用阿斯匹林降低结直肠癌的风险，但是阿斯匹林影响肠道致癌作用的机制并不是很清楚。
- The concentration of ADR was in LoVo/Adr cells was significantly lower than that in LoVo cells. LoVo/Adr细胞内阿霉素含量显著低于LoVo细胞 ,加入PMA后 ,Lo Vo/Adr细胞内ADR含量进一步减少 ;
- At present there is a little literature about BTW, and the effects that BTW have on LOVO cells have not been reported. 目前国内外关于BTW的研究报道不多，BTW对肠癌细胞LOVO的影响尚未见报道。
- BTW significantly inhibited the growth of LOVO cells in dose-dependent manner by flow cytometric analysis. FCM结果表明，BTW对LOVO细胞有较强的抑制作用，并存在着剂量依赖关系。
- Keywords Colorectal neoplasm;Genistein;Apoptosis;Cell cycle;ERK; 结肠肿瘤;金雀异黄素;细胞周期;凋亡;ERK;
- Aim:To observe the apoptosis induced by Calamistrin J on human colon carcinoma Lovo cells,and to explore its mechanism. 目的:观察刺果紫玉盘素J(Calamistrin J,Cal-J)诱导人结肠癌Lovo细胞凋亡的作用,并探讨其诱导细胞凋亡的可能机制。
- Cerulenin also inhibited the proliferation of LoVo cells in vitro and was dose-dependent. 同时诱发细胞发生凋亡。
- Keywords Colorectal neoplasm;Multidrug resistance;Cell subline; 关键词结直肠肿瘤;多药耐药;细胞株;
- The adherence of HUVEC cell line to LOVO cell line was detected by using the rose bengal strain. 孟加拉玫瑰红活细胞染色法观察arresten因子对内皮细胞与LOVO细胞黏附的影响。
- By AFM we have studied the induction of apoptosis LOVO cells with BTW and the preparation of Langmuir-Blodgett (LB) films. 本文主要用原子力显微镜研究了BTW诱导肠癌细胞LOVO的凋亡和LB膜的制备。
- AbstractAIM To study the diagnostic value of serum gastrin in patien ts with colorectal neoplasms. 摘 要目的 研究结直肠肿瘤患者血清胃泌素水平与病情的相关性.
- Methods LoVo cell line was cultured using nitrocellulose membrane as the culture medium, in parallel with the same cell culture on the coverglass which served as control. 方法应用硝酸纤维素膜培养细胞和病理染色并与常规方法培养细胞的比较。
- Objective: To investigate the value of Endoscopic Mucosal Resection(EMR) in dignosis and treatment of colorectal neoplastic lesions. 目的 :探讨内镜粘膜切除术 (Endoscopic Mucosal Resection:EMR)在结直肠肿瘤诊治中的临床应用价值。
- The combination of 5-fluorouracil(5FU) with p14ARF gene could increase the inhibitory function of 5FU and sensitize human colon cancer LOVO cell line to 5FU. pl4ARF基因和化疗药物5FU联合用于结肠癌LOVO细胞能够加强5FU的生长抑制作用，明显提高LOVO细胞对5FU的敏感性。
- Keywords colorectal neoplasm;narrow band imaging;chromoendoscopy; 结肠肿瘤;窄带内镜;色素内镜;
- FCM showed that LoVo cells treated with curcumin were accumulated in S, G_2 + M phase in the cell cycle, the ratio of cells in G_(0/1) phase was decreased in 24 hours of treatment with curcumin. 而G_(0/1)期的细胞比例减少。 处理48h后,G_2+M期的细胞比例反而降低,部分可能由于G_2+M期细胞并发凋亡所致。
- The human colon cancer LoVo cells were transfected by Lipofectamine 2000 with plasmid vector, and then selected for 4 weeks by G418. The expression of cerb-B1 mRNA was assessed by real time PCR and the protein of EGFR was assessed by Western blot method. 应用脂质体Lipofectamine2000转染人结肠癌LoVo细胞,转染成功后以G418筛选4周,实时荧光定量RT-PCR(RealTimeRT-PCR)检测cerb-B1基因的mRNA表达,Western-blot检测EGFR蛋白的表达。
