DNA sequencing indicated that the inserted fragment in pBluescript-SK-II vector was HNP-3 mature peptide gene sequence,and the sub-cloned recombinant pGBKT7-HNP-3 was no mismatch.

• 构建的重组质粒pBluescript-SK-II-HNP-3,经测序鉴定,插入片段为HNP-3成熟肽的cDNA序列。 亚克隆构建的重组诱饵质pGBKT7-HNP-3,测序鉴定表明其插入目的基因序列无误。