Dimeric AK was used as a model of oligomeric protein to investigate itsunfolding/refolding pathway in thermal or denaturant denaturation, with theaim to trap the possible equilibrium or kinetic intermediates.

• 在以海参 AK 为模型,探讨寡聚蛋白去折叠/再折叠机制时,本论文分别对AK 在热和变性剂条件下的可能去折叠/再折叠途径进行了研究,捕捉平衡态与动力学去折叠/折叠中间体。