Diosgenin was extractied with chloroform instead of circumfluence and then oxidized by Liebemann-Barcharad reagent and finally determined with a spectrophotometer.

• 提取甾体皂甙元时采用微量样品以氯仿浸提代替传统的回流抽提法，以Liebemann-Barcharad试剂显色，用分光光度法测定。