Experimental DVT was induced in 18 rats by block of blood in femoral vein with vascular clamp,then take out of thrombi and inject them into the other femoral veinein to make DVT-PTE model(54).

• Sham组依据上述相应时间分为十二组,每组6只。 手术阻滞实验组大鼠的左股静脉形成DVT,DVT造模成功后依据上述不同时间段取出股静脉栓子于右股静脉注入制成PTE模型。