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- Methods Enzyme linked immunosorbent assay (ELISA) and fluorescence quantitative polymerase chain reaction (FQ PCR) technique were used to detect TOX specific antibodies and TOX DNA in the blood of pregnant women to diagnose maternal infection. 方法 用酶联免疫吸附法及荧光定量多聚酶链反应技术检测孕妇血中 TOX特异性抗体及 TOX DNA,对 TOX感染孕妇检测羊水或脐血诊断胎儿感染。
- Objective To establish a PCR assay for the detection of Helicobacter pylori. 目的建立一种PCR方法检测幽门螺杆菌(Helicobacter pylori,Hp)感染。
- Objective To detect Plasmodium falciparum with the Fluorescent Quantitative PCR(FQ PCR) and value this method. 目的评价荧光定量聚合酶链式反应(FQ-PCR)检测恶性疟原虫的效果。
- Objective To establish PCR assay of Porcine Parvovirus detection in Trypsin. 目的建立原材料胰蛋白酶中猪细小病毒的PCR检测方法。
- Results The positive rate detected by microscopy, common PCR and FQ PCR is 33.1%?38.5% and 40.9%. 结果恶性疟原虫镜检、常规PCR、FQ-PCR检出的阳性率分别为33.;1%25、38
- Methods:The amount of HBV DNA in the sera of patients with chronic hepatitis B (CHB), chronic severe hepatitis B (CSHB) and hepatic B cirrhosis (HBC) were measured with FQ PCR. 方法 :采用荧光定量 PCR( FQ- PCR)法检测慢性乙型肝炎 ( CHB)、慢性乙型重型肝炎 ( CSHB)、乙型肝炎性肝硬化 ( HBC)患者血清 HBV DNA含量。
- Rapid detection of measles virus RNA by FQ - PCR assay 荧光定量-聚合酶链反应法快速检测麻疹病毒核酸
- Development and application of multiplex PCR assay for the detection of Campylobacter spp. 弯曲菌多重PCR检测方法的建立及其初步应用。
- PCR assay used to detect the conserved toxoplasmic genes also showed positive result. 应用PCR方法检测弓形虫保守基因，结果为阳性。
- It is indicated by PCR assay that the aim gene have been integra ted into maize genome. PCR检测证明，目的基因已整合到玉米基因组中。
- Methods Detecting SFGR DNA sequence fragment in spleen of rodents and ticks by PCR assay. 方法用PCR法检测鼠类脾脏和蜱类中的斑点热群立克次体DNA序列片段。
- PCR assay and Southern blot analysis showed that CMO gene was integrated successfully into the genome of transformed plant. PCR及Southern检测证明CMO基因已整合进抗性植株基因组;
- The mean HBV DNA level decreased 5.9 lg copies/ml(by PCR assay)from baseline in ETV group versus 4.3 lg copies/ml in LVD group(P<0.0001). HBVDNA的载量(PCR定量);ETV组平均下降5.;9lg拷贝/ml;LVD组平均下降4
- In addition, there were 2 negative samples in PPD test, while they were positive in nested PCR assay and also in FQ-PCR assay. 在对2份PPD检测阴性而巢式PCR检测阳性的临床样本的检测结果也为阳性。
- Conclusion The pandemic group-specific multiplex PCR assay can fast identify pandemic vibrio parahaemolyticus and providing a methodology for searching epidemic pathogen. 结论群特异多重PCR法能快速鉴定当前副溶血性弧菌流行群，有利于流行病学溯源等。
- Moreover,this PCR protocol can detect Cff sufficiently in 100 pg template DNA. It was demonstrated to be a highly sensitive,specific and rapid PCR assay for the detection of Cff. 该检测方法特异性强、灵敏度高 ,在 10 0pg的基因组DNA浓度下仍能检测到很强的条带
- The real-time PCR assay was evaluated using 60 B. cereus group strains and 28 others.The assay was also used to construct calibration curves for different food matrices and feces. 共使用60株仙人掌杆菌群菌株以及28株其他菌株进行即时聚合酶链反应方法之评估，并应用于不同之食品基质及粪便检体。
- Histologic improvement, preferably with HBV DNA levels not detectable by PCR assay, are probably more appropriate short-term endpoints for efficacy of treatment. 组织学的改善，最好伴随着病毒DNA无法检出（PCR法），可能时更合适的短期治疗终点。
- Objective In order to set up rapid identification for the pandemic vibrio parahaemolyticus,develope a pandemic group-specific multiplex PCR assay for base unit. 目的建立适于基层单位应用的针对副溶血性弧菌流行群的特异多重PCR方法。
- The established specific PCR assay should provide a practical and rapid method for the identification and differentiation of Eimeria species in mixed infections. 用本项研究所建立的特异PCR方法对这些混合感染的球虫种类进行鉴定具有一定的实用价值。
