First,we refer Blake method of preparation to make heat shock pretreatment model rats,after 24h, Anhydrous alcohol(2.5g/kg,99.7%) was give to Simple alcoholgroup and heat shock pretreatment add alcohol group by peritoneal injection.

• 热休克预处理加酒精组(参照Blake模型制备法)，用2q0戊巴比妥纳(40mg/kg)腹腔注射麻醉，再放入60℃恒温箱中，肛温达42℃后，持续巧min，取出室温25℃下继续饲养24h后，再予酒精处理，余具体步骤同单纯酒精组;