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- Conclusion The fluorogenic Quantitative PCR method for the detection of Plasmodium falciparum is simple and accurate. 结论 建立了检测恶性疟原虫的荧光定量PCR方法,较常规PCR技术更为简便、快速、准确,有很好的应用前景和研究价值。
- Objective To search the better molecular biology method in the detection of HCMV by comparing the role of common PCR with Fluorogenic Quantitative PCR (FQ-PCR). 目的比较常规PCR法与荧光定量PCR(FQ-PCR)法在人巨细胞病毒(HCMV)检测中的作用,寻找更适合于HCMV检测的方法。
- Methods The verification of EBE was based on detection of EBV DNA in cerebrospinal fluid (CSF) by fluorogenic quantitative PCR(FQ-PCR) and Nest-PCR. The clinical and CSF changes of 27 EBE cases and 26 controls were analyzed and compared. 方法 对 2 7例经荧光定量PCR(FQ PCR)及巢氏PCR方法检测脑脊液病毒阳性而确诊的EBE与 2 6例单纯疱疹病毒脑炎 (HSE)患儿临床及脑脊液资料进行回顾性分析。
- Chen YD, Zhang CY, Zou JX, et al.Development of a fluorogenic quantitative PCR assay for rapid quantification of hog cholera lapinized virus [ J ].Chin Virol Sin J, 2003, 18(2): 124-8. [12]陈玉栋;张楚瑜;邹俊煊;等.;建立快速定量检测猪瘟免化弱毒苗的荧光定量PCR技术[J]
- Fluorogenic quantitative PCR method for Plasmodium vivax 间日疟原虫荧光定量聚合酶链反应检测方法的建立
- Keywords Human cytomegalovirus;Breast milk: Fluorogenic quantitative PCR; 人巨细胞病毒;母乳;荧光定量PCR法;
- Development of a Fluorogenic Quantitative PCR Assay for Rapid Quantification of Hog cholera lapinized virus 建立快速定量检测猪瘟兔化弱毒苗的荧光定量PCR技术
- Detection of HPV DNA in patents with condyloma acuminatum using fluorogenic quantitative PCR technique 用核酸荧光定量PCR技术检测尖锐湿疣HPV的研究
- Keywords human papillomavirus;DNA load;real-time fluorogenic quantitative PCR;recurrence; 关键词人乳头瘤病毒;DNA载量;定量PCR;复发;
- Fluorogenic quantitative PCR 荧光定量PCR
- Methods CK19mRNA and CK20mRNA expression in peripheral blood were detected in 38 cass of esophageal cancer by fluorogenic quantitative RT-PCR. 方法用荧光定量RT-PCR方法检测38例食管癌患者外周血CK19mRNA和CK20mRNA表达。
- Fluorescence quantitative PCR combined with RT-PCR technique can detect the DTC in blood of the patients with HCC. RT-PCR联合荧光标记探针杂交法能够检测到肝癌患者血液中播散的肿瘤细胞,并且以肺动脉血中阳性率最高。
- For special aims, multiple PCR, reverse PCR, quantitative PCR, in situ PCR, as well as capillary PCR, were developed. 而适合不同目的的PCR技术也得到了充分的发展,如多重PCR、反转录PCR、定量PCR、原位PCR、PCR突变、毛细管PCR技术等等。
- The expression of RAE-1 and H60 genes was measured by RT-PCR and real-time quantitative PCR. 6) BALB/C小鼠正常肝脏组织能检测到RAE一1低表达。
- Objective To detect Plasmodium falciparum with the Fluorescent Quantitative PCR(FQ PCR) and value this method. 目的评价荧光定量聚合酶链式反应(FQ-PCR)检测恶性疟原虫的效果。
- Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis. 目的探讨荧光定量聚合酶链反应(FQ-PCR)技术诊断结核病临床应用价值。
- Fluorescent real-time quantitative PCR was used to investigate some of the differentially expressed genes. 对部分筛选出来的差异表达基因使用荧光定量PCR方法检测并确认其变化;
- Conclusions Quantitative PCR is a practical method for diagnosing DMD/BMD carrier with great rapidity、accura... 结论定量PCR方法是一种准确、快速、简便的DMD携带者诊断方法。
- Objective:To construct recombinant plasmid pMD18-nm23-H1/p as the standards for nm23-H1 gene detection by real-time fluorescence quantitative PCR. 目的:制备用于nm23-H1基因mRNA实时荧光定量PCR检测的质粒标准品。
- Methods The HBV-DNA was detected by florescence quantitative PCR and HBV serum markers was detected by ELISA in 392 serum samples. 方法392份血清用酶联免疫吸附试验(ELISA)法测定乙肝病毒(HBV)两对半,用FQ-PCR法检测HBV-DNA含量。