GA4-Ovalbumin (OVA) is synthesized via 1- ethyl-3-(3 dimethylaminopropyl-) -carbodiimide hydrochloride (EDC). An indirect enzyme-linked-immunosorbent-assay (ELISA) is developed for the quantitation of GA1, 3,4,7,utilizing GA4-OVA as a coating agent.

• 用碳二亚胺(EDC)法将赤霉素A_4(GA_4)与卵清蛋白(OVA)连接；形成GA_4-OVA复合物用于包板；利用GA_4-Me与GA_4-OVA对兔抗GA_4-PAbs竞争结合反应；建立了GA_(1；3；4；7)间接酶联免疫检测法(ELISA).