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- GFP expressing cassette 黄色荧光蛋白（YFP）表达盒
- Methods BTT-T739 cells, a mouse bladder transitional cell carcinoma cell line, were tranfected with GFP plasmid to screen stable GFP expressing clones. 方法 将绿色荧光蛋白 (GFP)表达质粒导入膀胱癌细胞株BTT T739,筛选出高表达GFP的细胞克隆并配置成细胞悬液。
- The results from RT-PCR indicated the knockdown of mRNA level of hCAP-E and hCAP-C in the co-transfeted Hela cells. We also conducted the co-transfection with the RNAi plasmids RHE and RHC and the selective marker GFP expressing plasmids simultaneously. 进行RT一PCR检测表明人集缩素SMC亚基hCAP一E和hCAP一C的C一末端真核表达质粒在Hela细胞内得到过表达。
- For example, GFP expressed in cells can yield a bright green fluorescence when the cells are excited by blue or UV light. 譬如，GFP在细胞中表达，在蓝光或紫外光照射下可以产生明亮的绿色荧光。
- Both genes were cloned into GFP expression vector pIRES2 EGFP and transfected into HeLa cells. 将上述基因克隆入绿色荧光蛋白(GFP)表达载体pIRES2-EGFP中,转染人HeLa细胞,在荧光显微镜和电镜下观察转染细胞的形态和结构。
- Results GFP expression could be observed on the third day after packing of the linearized pAdIL-10 in 293 cells. 结果 pAdIL-10经293细胞包装，3d后可观察到绿色荧光蛋白(GFP)表达；
- The expression cassette OAAT for multiple-epitope antigen of FMDV was successfully designed,and trivalent FMDV DNA vaccine pVAX1-OAAT was successfully constructed. 已成功设计FMDV复合多表位基因工程疫苗表达盒OAAT,并构建了三价口蹄疫核酸疫苗pVAX1-OAAT。
- The invention further relates to an expression cassette comprising a useful polynucleotide placed under the control of a nucleic acid such as defined above. 本发明还涉及包含置于如上文所述核酸控制之下的目的多核苷酸的表达盒。
- GFP expression in co-transfected AAV-293 cells was observed and the titer of rAAV is 106particles/ml measured by that cells expressing green fluorescence were counted and compared with the total number of cells.. 2
- However,until now,little is known about the function of pseudorabies virus(PRV) UL14.In this study,an UL14-negative PRV mutant(PRV-UL14D) was constructed by inserting an EGFP expression cassette into the UL14 coding sequence. 本研究以伪狂犬病毒鄂A株(PRV-Ea)为亲本株,通过同源重组,将编码EGFP的真核表达盒插入到伪狂犬病毒基因组的UL14基因中,PCR检测和荧光观察证实获得了能稳定表达EGFP的UL14插入失活突变株PRV-UL14D。
- In the optimum condition: FuGENE 6:DNA=6:1, transfection of rat MSC withGFP displayed a stable and long-term GFP expression in vitro, and untransfectedMSC gradually died in G418 selecting culture. 在FuGENE6:DNA二6:1的优化条件下,MSC能在体外长期稳定的表达绿色荧光蛋白,未转染上的细胞在G418的筛选下逐渐死亡。
- Methods Green fluo rescence protein(GFP)gene was used as a reporter gene,and was transferred int o lens epithelial cells mediated by retroviral vector,GFP expression in cells w as analyzed. 方法用绿色荧光蛋白基因作为报告基因,以逆转录病毒载体携之转染晶状体上皮细胞,观察GFP在晶状体上皮细胞的表达情况。
- He bought a new cassette recorder. 他买了一台新的盒式录音机。
- Our results indicate that the irradiation can effectively control adenoviral-mediated GFP expression in tumor cells via Egr-1 promoter, and these data laid basis for further gene radiotherapy study. 结果说明，放射经Egr-1启动子可有效调控腺病毒介导gfp基因的肿瘤细胞内表达。
- He was enthusing about his new pocket cassette recorder. 他对自己的新袖珍录音机爱不释手。
- Cassette tape recorders are out of date now. 现在，盒式磁带录音机过时了。
- Cassette recorder is not allowed in the concert. 录音机不准带入音乐会会场。
- The cassette player is an optional extra in this make of car. 这种型号汽车如需盒式磁带唱机费用另计。
- No words can express the grandeur of that parade. 阅兵式那宏伟的场面是无法用语言表达的。
- It is better to express your anger, rather than bottle it up. 生气宁可发泄出来而不要闷在肚内。
