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- Using genomic PCR and sequencing, FLT3/ITD mutation with or without chromosome translocation were examined in AML patients. 采用PCR联合序列检测伴有或不伴有染色体易位的急性髓性白血病患者FLT3基因突变情况。
- Methods Reverse transcription polymerase chain reaction (RT-PCR) and genomic PCR were applied to monitor FLT3/ITD mutation in 126 AML patients. 方法采用基因组PCR及RT-PCR方法检测126例AML患者的FLT3/ITD突变。
- Genomic PCR 基因组PCR
- Vein blood samples 4-6ml were collected and Genomic DNA were extracted. Exon 9 of CFH gene was PCR amplified . 抽取外周抗凝血4-6ml,用酚-氯仿抽提法提取全基因组DNA,聚合酶链反应(Polymerase chain reaction ,PCR)扩增外显子9片断并进行直接测序。
- In order to determine the genotype of the mice by PCR,genomic DNA of LPL(-/-) knock-out mice was extracted from tails. 采用从LPL(-/-)基因敲除小鼠的个体组织(鼠尾)中提取基因组DNA进行PCR分析,对其LPL(-/-)基因型作出鉴定。
- The zot gene encoding Zonula occludens toxin was amplified from classic Vibrio cholerae genomic DNA by PCR. 从霍乱疫苗菌中抽提基因组DNA,用PCR的方法扩增zot基因。
- Extract the genomic DNA of a BLAB/c mice . 用酚氯仿异戊醇抽提blab/c鼠全基因组DNA;
- The PCR detection of karnal bunt of wheat. 小麦印度腥黑穗病菌PCR检测。
- The size of the amplified PCR product was 822 bp. PCR扩增片段大小为822bp,最小检测量为1080个HPs。
- Ni var mitt liv--jag lever genom er! (你们是我的生命,我通过你们而活着。
- The ermA and ermC genes were amplified by PCR. PCR检测与诱导型克林霉素耐药相关的ermA、ermC基因。
- G-LCR detected ten-fold EBs than PCR. G -LCR较PCR敏感 10倍 ;
- Analyse the genotype of the target genes using PCR. 聚合酶链反应(PCR)扩增目的基因进行基因型分析。
- C3d-P28 coding gene was amplified by PCR. 将PCR获得的补体C3d-P28 DNA序列以头尾串连的方式构建四聚体。
- PCR, when you need to solve a crime. 当你需要知道谁是孩子的爹时,
- Methods:Urease test and PCR were used. 方法:采用脲酶实验及PCR方法检测幽门螺杆菌。
- PCR products were sequenced and analyzed. PCR产物测序及分析。
- It is concordant with routine PCR method. 并与常规PCR法具有很好的一致性。
- COI genes of hookworm was amplified from hookworm genomic DNA by PCR and sequenced. Program MEGA was used for the sequence analysis. 采集安徽和四川两省的十二指肠钩虫 ,用PCR技术扩增COI基因 ,同时测序并用MEGA软件进行遗传差异的分析。
- Historical Review on the Genomic Denomination of the Genus Oryza L. 植物染色体组命名的历史回顾。