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- There was no correlation between HCV RNA load and ALT activity. HCV RNA拷贝量与ALT活性无相关性。
- And all patients were evaluated based on seronegative of HCV RNA. 采用AmpliSensorTM HCV RNA荧光定量试剂盒对所有患者进行HCV RNA定量检测。
- Treat the MOLT-4 cells with anti-HCV antibody and HCV RNA positive and negative serum, respectively. 用抗HCV抗体和HCVRNA阳性及阴性血清感染MOLT-4细胞，制备细胞片进行免疫酶染色。
- Objective To evaluate the relationship of Hepatitis C Virus RNA(HCV RNA) and Antibody of HCV(Anti-HCV) and Alannine Aminotransferase (ALT). 目的了解丙型肝炎病毒核糖核酸(HCV RNA)与抗丙肝病毒抗体(抗-HCV)及丙氨酸氨基转移酶(ALT)的关系。
- Objective:To analyse the relationship of HCV RNA titre,ALT,AST and cytokines secreted by PBMCs in vitro in patients with chronic HCV infection. 目的:研究慢性HCV感染患者HCV RNA水平、ALT、AST以及外周血单个核细胞(PBMCs)体外细胞因子分泌之间的关系。
- The expression of FAS antigens in PBMC and in the liver tissue are correlated to liver inflammation and serum HCV RNA in patients with CHC. ( 1)CHC患者肝组织和PBMC中FAS表达与肝组织炎症及血清病毒载量有关 ;
- Methods EIA was established with HCVE2/NS1 relative conservation polypeptide antigen, as well as serum HCV RNA and ALT in patients with HCV infection. 方法利用HCVE2/NS1基因编码的膜区糖蛋白合成E2/NS1相对保守区多肽抗原，建立酶免疫试验（EIA），对96例HCV感染者及40例正常献血员进行抗HCVE2/NS1抗体的检测，同时平行检测HCVRNA和丙氨酸转氨酶（ALT）。
- Most of the patients with HCV RNA positive were positive for RIBA,only 3 cases were indeterminate, no one was negative for RIBA. HCVRNA阳性病例中RIBA多为阳性，可疑仅占5.;36%25（3/56），无1例阴性。
- The cDNA probe hybridized with BVDV RNA, HCV RNA and yeast tRNA by the dot blot method The BVDV RNA and HCV RNA were positive and yeast tRNA was negative. 琼脂糖凝胶电泳结果说明它与BVDV RNA相似。 该cDNA的探针用斑点杂交方法与BVDV RNA,HCV RNA和酵母tRNA杂交,BVDV RNA和HCVRNA显阳性反应,酵母tRNA显阴性反应,结果说明合成的cDNA为BVDV RNA的cDNA。
- The serum ferritin was determined prior to treatment,and then ALT and HCV RNA levels in serum were determined at 0,12,24,48 wk during the antiviral treatment. 分别在0,12,24及48 wk检测血清ALT及HCV RNA以判断疗效。
- The ALT levels and HCV RNA concentrations of patients were detected after 12 weeks of treatment. EVR and BR rates of patients in different serotypes were compared. 治疗12周时检测患者的丙氨酸氨基转移酶(ALT)和HCVRNA,观察患者EVR和BR。
- Moreover, antiretroviral therapy used to suppress HIV replication is often associated with a paradoxical increase in HCV RNA levels, as well as hepatotoxicity. 此外，抗逆转录病毒治疗用于抑制HIV复制通常与HCV RNA反常升高和肝中毒相关。
- Maximal antiviral effects were observed at approximately study day four, when peak mean reductions in HCV RNA ranged from 0.3 to 1.4 log10 (50 to 96 percent) across all doses. 最大的杀病毒的效果在大约研究日子四被观察; 当在 HCV RNA 的最高的低劣减少横过所有的剂量从 0.;3 到 1
- Inclusion criteria in this trial include documented history of HCV infection for greater than 6 months, and HCV RNA levels of greater than 104 IU/mL at the screening evaluation. 这试验的内含物标准在筛检评价包括为大于 6个月 , 和大于 104 IU/毫升的 HCV RNA 水平证明 HCV 感染的历史。
- OnlY 59%(13/22) of sera positive with antiHCV by ELISA were shown to be positive by the second generation recotnbinant immunoblot assay(4.RIBA ) and 60%was positive with HCV RNA by reverse tran-scription-polymerase chain reaction(RT-pCR). 阳性血清经重组免疫印迹法（4·RIBA）鉴定仅59%25阳性，用逆转录聚合酶链反应检测HCVRNA阳性率仅60%25；
- Though HCV RNA amplification by PCR is the most sensitive and specific method for the detection of HCV, the complicated manipulation, easy contamination and high cost prevented PCR from application in a large scale. PCR检测HCV RNA是目前HCV诊断最灵敏、特异的方法，但其操作比较复杂，易造成污染，且价格昂贵，不适于大规模的筛查应用。
- Methods FAS antigen in PBMC and in the liver of patients with CHC were detected by immuno-histochemistry and serum HCV RNA were detected by PCR. Liver tissue section and serum ALT were detected simultaneously. 方法 采用肝活检组织病理、免疫组织化学、PCR及血液生化等方法检测 41例CHC患者的肝组织和PBMC中FAS抗原、血清HCVRNA及肝功能 ,观察肝组织和PBMC中FAS抗原表达情况与血清HCVRNA水平及肝损伤的关系。
- Detecting HCV RNA by one-step short-product RT-PCR 一步短片段RT-PCR方法检测HCV rna
- To analyze genotype of HCV RNA with gene chip 基因芯片法对丙型肝炎病毒RNA的基因型分析
- Quantive detection of HCV RNA by competitive RT PCR 竞争性逆转录聚合酶链反应定量检测对HCV RNA
