Hypothalamic total protein (TP) of rats in each group were separated by immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE), and PDQuest 7.0 software was applied to analyse 2-DE images after coomassie blue staining.

• 用固相pH梯度双向凝胶电泳分离各组大鼠下丘脑的总蛋白质；经考马斯亮兰染色；PDQuest7.；0软件分析。