If under asepsis condition,without mixed antibiotic,germ growth was not influenced and bacteria pollution was not caused. 2.Liquid cultivation media for Hp: Bromfield medium added with mixed antibiotic and 6% horse serum could promote proliferation of Hp.

• 在严格无菌的条件下,不添加混合抗生素,培养基为哥伦比亚琼脂+6%25马血清,对细菌的生长没有影响,且不会造成杂菌污染(。2)采用液体培养hp时,培养基为布氏肉汤基础加混合抗生素和6%25马血清,hp增菌迅速。