In a subset of experiments, in io AR was additionally measured in orotracheally intubated, spontaneously breathing mice 24 h after Penh measurement, and lungs were isolated further 24 h later.

• 在一个亚组实验中，Penh测定后24小时后，小鼠经口气管插管、自主呼吸状态下测定气道反应性，再经过24小时，游离肺组织。