In chloroplast, this enzyme consists of two parts: membrane-embedded CFo and cytoplasmic CF1. CFo conducts protons flux through the thylakoid membrane and provides the binding site for CF1. CF1 contains the nucleotide-binding and catalytic sites.

• CFo形成跨膜的质子通道,并为CF1提供膜上的结合位点。 CF1上具有核苷酸结合和催化位点。