In order to elucidate the location of chorismate mutase and prephenate dehydrogenase on E. coli T-protein, protein limited digestion and fragmentation cloning were employed.

• 摘要为了解析分支酸变位酶和预苯酸脱氢酶在大肠杆菌T蛋白的定位，根据T蛋白限制性水解结果，分段克隆分支酸变位酶和预苯酸脱氢酶。