In order to study the effect of iron on the expression of UCP2 in white fat and UCP3 in muscle in obese rats, a reverse transcription polymerase chain reaction (RT PCR) technique is used to measure the expression of UCP2 and UCP3 mRNA.

• 为了探讨铁对肥胖大鼠白色脂肪组织中UCP2及骨骼肌中UCP3基因表达的影响 ,应用RT PCR方法测定UCP2、UCP3mRNA表达水平。