In the establishment of flow cytometryassay of determination of cytotoxicity, the test principle was based on target celllabeling with CFSE and subsequent DNA-labelling with PI for identification oftarget cells with compromised cell membranes.

• 流式细胞术检测细胞毒的方法基于单细胞水平,克服了上述方法的缺陷,实验原理是先用第一种荧光染料标记靶细胞,用以区分效应细胞和靶细胞。 再用第二种DNA 荧光染料来区分膜已破坏的靶细胞并计算其百分比。